Screening of Ultraviolet-Induced Thermotolerant Yeast Mutants and Their Performance

The simultaneous saccharification and fermentation (SSF) technique holds promise for the conversion of lignocellulose to ethanol. However, optimal temperature yeast is lower than enzymatic hydrolysis process, which leads actual production process SSF usually being 38 °C. In this work, two ultraviolet (UV)-induced mutations were performed step by using Saccharomyces cerevisiae BY4742 as original strain enable perform well at higher temperatures. Thermotolerant strains obtained through mutagenesis screening, YUV1-1 YUV2-2, utilized a targeted 40 They ethanol yields comparable those °C in SSF, whereas decreased about 10% compared This study proves that thermotolerant adapted elevated temperatures can be UV thereby increasing stability processes lowering subsequent distillation costs.